Application note No. AN1012IL02
Experiments and image processing:
Joe Yu1), Paul Webster 1), Ben Leung 1), Kevin Mortimer1), Logan Wright1)
Report written by:
Joe Yu1) (edited by James Fraser1))
1) Queen’s University
DNA, amino acids, peptides and protein analysis, profiling saccharides, pollutants, nerve agents, and explosives screening, electrophoretic separation of ions, and detection of purines
Current methods of fabrication: hot embossing, room temperature imprinting, injection molding, laser ablation, in situ polymerization and solvent etching. Chen et al. [Eletrophoresis. Fabrication, modification, and application of poly(methyl methacrylate) microfluidic chips, 29: 1801-1814 (2008)] gives an overview of applications and fabrication techniques.
Specifically, Sun et at. [J. Micromech. Microeng. Low-pressure, high-temperature thermal bonding of polymeric microfluidic devices and their applications for electrophoretic separation, 16: 1681-1688 (2006)] use a CO2 laser with varying power and scan speeds. Showcased is a trench cut at 0.75 W average power and a scan speed of 32 mm/s giving a volumetric removal rate of 120 μm3/μsec. Feature size, heat affected zone and processing speed are all important considerations for the application.
Ekspla PL10100 (centre wavelength: 1064 nm, repetition rate: 50 kHz). Patterning done with galvo scanning.
EKSPLA note: due to the continuous product improvements, laser models were replaced respectively: NL640 and NL15100 were replaced by Baltic and BalticHP series, PL10100 was replaced by Atlantic series.
In situ imaging
White light interferometer centred at 800 nm (“OCT M mode”) with image line rates up to 312 kHz (axial image).
Ex situ imaging
Bright field microscopy.